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SUBCELLULAR LOCATION

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Main location(s)

Nucleoli, Nucleus but not nucleoli, Cytoplasm

Additional location(s)

 

Staining summary

Localized to the nucleus but excluded from the nucleoli or localized to the nucleoli and cytoplasm.

Reliability (APE)

Very low

Antibodies in assay

CAB004643, HPA012385, HPA017287
 

Antibody CAB004643

 

Antibody HPA012385

 

Antibody HPA017287

 

         Cell line A-431

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Location(s)

 Nucleoli, Cytoplasm Nucleus but not nucleoli 

Staining characteristics

 Granular  

Staining intensity

Negative Weak Weak 

Validation

No staining.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 

Comment

   

         Cell line U-2 OS

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Location(s)

 Nucleoli, Cytoplasm Nucleus but not nucleoli 

Staining characteristics

   

Staining intensity

Negative Weak Weak 

Validation

No staining.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 

Comment

   

         Cell line U-251 MG

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Location(s)

 Cytoplasm Nucleus but not nucleoli 

Staining characteristics

 Granular  

Staining intensity

Negative Weak Weak 

Validation

No staining.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 

Comment

   

         Antibody summary


Immunofluorescent staining of human cell line A-431 shows no positivity.
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleoli & cytoplasm.
 

Immunofluorescent staining of human cell line U-251 MG shows positivity in nucleus but not nucleoli.
 

Summary

No staining was observed in any of the three cell lines.   Staining of cytoplasm in all three cell lines with additional staining of nucleoli in A-431 and U-2 OS.   Staining of nucleus in all three cell lines.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Cytoplasm
Nucleoli
Nucleus but not nucleoli