BMP2K

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA026436

 

Antibody HPA026451

 

Antibody HPA026501

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 Atlas Antibodies
Sigma-Aldrich
 Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA026436 HPA026451 HPA026501 

Host species

Rabbit Rabbit Rabbit 

Clonality

pAb pAb pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand Affinity purified using the PrEST-antigen as affinity ligand Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

6 6 6 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment Recombinant protein fragment Recombinant protein fragment 

Length (aa)

82 96 79 

Antigen sequence

FISHSGSPEKKAEHSSINQENGTANPIKNGKTSPASKDQRTGKKTSVQGQ
VQKGNDESESDFESDPPSPKSSEEEEQDDEEV
 
HHHHLLQDAYMQQYQHATQQQQMLQQQFLMHSVYQPQPSASQYPTMMPQY
QQAFFQQQMLAQHQPSQQQASPEYLTSPQEFSPALVSYTSSLPAQV
 
YSANRSVADKEAIANFTNQKNISNPPDMSGWNPFGEDNFSKLTEEELLDR
EFDLLRSNRLEERASSDKNVDSLSAPHNH
 

Matching transcripts

BMP2K-003 - ENSP00000424668 [100%]
BMP2K-201 - ENSP00000334836 [100%]
 BMP2K-001 - ENSP00000421768 [100%]
BMP2K-003 - ENSP00000424668 [100%]
BMP2K-201 - ENSP00000334836 [100%]
 BMP2K-003 - ENSP00000424668 [100%]
BMP2K-201 - ENSP00000334836 [100%]
 

Other gene match

   

ANTIBODY VALIDATION

         Immunohistochemistry

Image

   

Description

Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity with a granular pattern in exocrine and endocrine cells.
More information
 Immunohistochemical staining of human duodenum shows strong cytoplasmic and nuclear positivity in glandular cells.
More information
 Immunohistochemical staining of human placenta shows strong cytoplasmic positivity in decidual cells.
More information
 

Retrieval method

HIER pH6 HIER pH6 HIER pH6 

Antibody dilution

1:175 1:50 1:600 

Validation IH

Uncertain: Two (or more) antibodies yielding dissimilar or partly similar staining patterns and there is no available gene/protein characterization data or only limited gene/protein characterization data Uncertain: Two (or more) antibodies yielding dissimilar or partly similar staining patterns and there is no available gene/protein characterization data or only limited gene/protein characterization data Uncertain: Two (or more) antibodies yielding dissimilar or partly similar staining patterns and there is no available gene/protein characterization data or only limited gene/protein characterization data 

         Western Blot

Image

   

Description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT-4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
   

Target mass (kDa)

129.2, 95.5 129.2, 95.5, 73.8 129.2, 95.5 

Antibody dilution

1:250 1:250 1:250 

Validation WB

Uncertain: Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data Non-supportive: Weak band of predicted size but with additional bands of higher intensity also present Non-supportive: Only bands not corresponding to the predicted size 

         Protein array

Image

   

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:3000 1:3000 1:3000 

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen. Uncertain: Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%). Supportive: Pass with single peak corresponding to interaction only with its own antigen. 

         Immunofluorescence

Image

   

Description

 Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus but not nucleoli.
More information
  

Antibody dilution

 1:50  

Validation IF

 Supportive: The subcellular location is supported by literature.  
 

ANTIGEN VIEW

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BMP2K-001
 
BMP2K-003
 
BMP2K-201