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SUBCELLULAR LOCATION

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Main location(s)

Nucleus but not nucleoli

Additional location(s)

Nucleoli, Cytoplasm 

Staining summary

Staining of cytoplasm A-431, nucleus in U-2 OS and both cytoplasm and nucleus in U-251 MG.

Reliability (Single)

Antibodies in assay

HPA005128
 

Antibody HPA005128

 

         Cell line A-431

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Location(s)

Cytoplasm 

Staining characteristics

Granular 

Staining intensity

Moderate 

Validation

Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 

Comment

 

         Cell line U-2 OS

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Location(s)

Nucleus but not nucleoli 

Staining characteristics

Granular 

Staining intensity

Moderate 

Validation

Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 

Comment

 

         Cell line U-251 MG

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Location(s)

Nucleoli, Nucleus but not nucleoli, Cytoplasm 

Staining characteristics

Spotty 

Staining intensity

Weak 

Validation

Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 

Comment

 

         Antibody summary


Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus but not nucleoli.
 

Summary

Staining of cytoplasm A-431, nucleus in U-2 OS and both cytoplasm and nucleus in U-251 MG.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Cytoplasm
Nucleoli
Nucleus but not nucleoli