The normal tissue page shows antibody staining and, when available, knowledge-based annotated protein expression in 46 human tissues. The assay and annotation is described here.
This page starts with a summary. The summary data can be based on one or more antibodies (reported by the "Antibodies in assay"-field). If the data is based on one single antibody, the IH validation score for that antibody is reported in the "Reliability (Single)"-field (color-coding described here). If the data is based on more than one antibody, the reliability score for the data is reported as "Reliability (APE)" (color-coding described here).
The tissues and cell types can be ordered by organ, cell type or alphabetically.
For each tissue and cell type, the antibody staining is given with the yellow-scale color-coding (described by the scale in the box to the right). Each available antibody is listed in a separate column and the antibody identifier is available at the bottom of the tissue list. The images and annotations can be accessed by clicking on the tissue name.
When available, a knowledge-based annotated protein expression (APE) is given to the right of the antibody staining values. The level of expression is given by a blue-scale color-coding (described by the scale in the box to the right).
At the very bottom of this page, a summary of the staining from each antibody is given, together with a representative image and the immunohistochemistry validation score for the antibody.
Immunohistochemical staining of human small intestine shows strong cytoplasmic and nuclear positivity in glandular cells.
Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human colon shows strong nuclear and cytoplasmic positivity in glandular cells.
Summary
Cells of the gastrointestinal tract, urinary bladder and fallopian tube displayed strong cytoplasmic and nuclear staining. Lymphoid tissues were in general moderately stained. Most other normal tissues were negative.
Glandular cells of gastrointestinal tract and surface epithelial cells of the urinary bladder were strongly positive. Cytotrophoblasts of the placenta, renal tubules and salivary ducts showed moderate immunoreactivity. Remaining normal tissues were generally negative.
The gastrointestinal tract, urothelium and ovarian follicle cells showed strong cytoplasmic and nuclear positivity. Cells in exocrine pancreas, renal tubules and gall bladder displayed moderate cytoplasmic immunoreactivity. Remaining normal tissues were generally negative.
Validation
Two (or more) antibodies yielding similar staining patterns which are consistent with available gene/protein characterization data
Two (or more) antibodies yielding similar staining patterns which are consistent with available gene/protein characterization data
Two (or more) antibodies yielding similar staining patterns which are consistent with available gene/protein characterization data
