The normal tissue page shows antibody staining and, when available, knowledge-based annotated protein expression in 46 human tissues. The assay and annotation is described here.
This page starts with a summary. The summary data can be based on one or more antibodies (reported by the "Antibodies in assay"-field). If the data is based on one single antibody, the IH validation score for that antibody is reported in the "Reliability (Single)"-field (color-coding described here). If the data is based on more than one antibody, the reliability score for the data is reported as "Reliability (APE)" (color-coding described here).
The tissues and cell types can be ordered by organ, cell type or alphabetically.
For each tissue and cell type, the antibody staining is given with the yellow-scale color-coding (described by the scale in the box to the right). Each available antibody is listed in a separate column and the antibody identifier is available at the bottom of the tissue list. The images and annotations can be accessed by clicking on the tissue name.
When available, a knowledge-based annotated protein expression (APE) is given to the right of the antibody staining values. The level of expression is given by a blue-scale color-coding (described by the scale in the box to the right).
At the very bottom of this page, a summary of the staining from each antibody is given, together with a representative image and the immunohistochemistry validation score for the antibody.
Immunohistochemical staining of human colon shows strong cytoplasmic positivity with a granular pattern, in glandular cells.
Immunohistochemical staining of human colon shows strong cytoplasmic positivity in granular pattern in glandular cells.
Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in granular pattern in glandular cells.
Immunohistochemical staining of human colon shows strong cytoplasmic positivity with a granular pattern in glandular cells.
Summary
A majority of normal tissues displayed moderate to strong cytoplasmic immunoreactivity with a granular pattern. Cells of the CNS were weakly stained.
Most of the normal tissues showed weak to moderate cytoplasmic positivity. Strong positivity in granular pattern was seen in cells of gastro-intestinal tract, adrenal gland, tubular cells of kidney and heart muscle. Lymphoid tissues and squamous epithelia were negative.
Most normal tissues were negative. Strong cytoplasmic positivity was observed in gastrointestinal tract and subsets of tubular cells in kidney. Testis, airway epithelia, urinary bladder and trophoblastic cells showed moderate cytoplasmic positivity.
Moderate to strong cytoplasmic positivity with a granular pattern was observed in most normal tissues. Gastrointestinal cells, renal tubules and heart muscle among other showed strong immunoreactivity.
Validation
Two (or more) antibodies yielding similar staining patterns which are consistent with available gene/protein characterization data
Two (or more) antibodies yielding similar staining patterns which are consistent with available gene/protein characterization data
Two (or more) antibodies yielding similar staining patterns which are consistent with available gene/protein characterization data
Two (or more) antibodies yielding similar staining patterns which are consistent with available gene/protein characterization data
