CLTC

ANTIBODY AND ANTIGEN INFORMATION

? »
 

Antibody CAB010389

 

Antibody CAB011571

 

Antibody CAB017155

 

ANTIBODY INFORMATION

Provider

Santa Cruz Biotechnology
 Abcam plc
 Kazusa DNA Research Institute
 

Product name

sc-9069 ab21679 GX0111 

Host species

Rabbit Rabbit Rabbit 

Clonality

pAb msAb pAb 

Purity

Protein A/G Affinity Not known 

Released in version

4 3 4 

ANTIGEN INFORMATION

Antigen

Recombinant protein Synthetic peptide Recombinant protein 

Length (aa)

   

Antigen sequence

 
 
 

Matching transcripts

   

Other gene match

   

ANTIBODY VALIDATION

         Immunohistochemistry

Image

   

Description

Immunohistochemical staining of human testis shows cytoplasmic positivity in cells of seminiferus ducts.
More information
 Immunohistochemical staining of human epididymis shows distinct cytoplasmic positivity in glandular cells.
More information
 Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in glandular cells.
More information
 

Retrieval method

HIER pH6 HIER pH6 HIER pH6 

Antibody dilution

1:50 1:7000 1:1000 

Validation IH

Uncertain: Two (or more) antibodies yielding partly similar staining patterns which are partly consistent with gene/protein characterization data or consistent with limited gene/protein characterization data Supportive: One antibody yielding a staining pattern which is consistent with gene/protein characterization data Uncertain: Two (or more) antibodies yielding partly similar staining patterns which are partly consistent with gene/protein characterization data or consistent with limited gene/protein characterization data 

         Western Blot

Image

   

Description

Lane 1: Marker [kDa] 213, 118, 83, 50, 33, 26, 18
Lane 2: RT-4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 Lane 1: Marker [kDa] 219, 111, 83, 48, 32, 26, 17
Lane 2: RT-4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT-4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

191.6, 187.9, 70.7, 25.7, 15.5, 13.7, 10.6, 2.4 191.6, 187.9, 70.7, 25.7, 15.5, 13.7, 10.6, 2.4 191.6, 187.9, 70.7, 25.7, 15.5, 13.7, 10.6, 2.4 

Antibody dilution

1:500 1:500 1:500 

Validation WB

Uncertain: No bands detected Supportive: Single band corresponding to the predicted size in kDa (+/-20%) Supportive: Band of predicted size in kDa (+/-20%) with additional bands present 

         Immunofluorescence

Image

   

Description

 Immunofluorescent staining of human cell line U-2 OS shows positivity in cytoplasm.
More information
 Immunofluorescent staining of human cell line U-251 MG shows positivity in cytoplasm.
More information
 

Antibody dilution

 1:2000 1:200 

Validation IF

 Supportive: The subcellular location is supported by literature. Supportive: The subcellular location is supported by literature. 
 

ANTIGEN VIEW

? »
 Not available