Fields »

SUBCELLULAR LOCATION

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Main location(s)

Nucleus but not nucleoli

Additional location(s)

Cytoplasm, Vesicles, Aggresome 

Staining summary

Mainly localized to the nucleus.

Reliability (APE)

High

Antibodies in assay

HPA041885, HPA042231
 

Antibody HPA041885

 

Antibody HPA042231

 

         Cell line A-431

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Location(s)

Nucleus but not nucleoli   

Staining characteristics

   

Staining intensity

Weak   

Validation

One/multiple location(s) supported by experimental gene/protein characterization data.
   

Comment

   

         Cell line HEK 293

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Location(s)

  Nucleus but not nucleoli, Aggresome 

Staining characteristics

   

Staining intensity

  Weak 

Validation

  
Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

   

         Cell line U-2 OS

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Location(s)

Nucleus but not nucleoli, Cytoplasm Vesicles 

Staining characteristics

  

Staining intensity

Weak Moderate 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data.
 
Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

  

         Cell line U-251 MG

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Location(s)

Nucleus but not nucleoli   

Staining characteristics

   

Staining intensity

Weak   

Validation

One/multiple location(s) supported by experimental gene/protein characterization data.
   

Comment

   

         Antibody summary


Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus but not nucleoli & cytoplasm.
 

Immunofluorescent staining of human cell line HEK 293 shows positivity in nucleus but not nucleoli & aggresome.
 

Summary

Staining of nucleus in all three cell lines with additional staining of cytoplasm in U-2 OS.   Staining of nucleus and aggresome in HEK 293. Stianing of vesicles in U-2 OS.  

Validation

The subcellular location is supported by literature.
 
The subcellular location is supported by literature.
 

Dictionary

Cell overview
Cytoplasm
Nucleus but not nucleoli
Endosome