SCYL1

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA015015

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA015015 

Host species

Rabbit 

Clonality

pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

4 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment 

Length (aa)

128 

Antigen sequence

AFPEDFCRHKVLPQLLTAFEFGNAGAVVLTPLFKVGKFLSAEEYQQKIIP
VVVKMFSSTDRAMRIRLLQQMEQFIQYLDEPTVNTQIFPHVVHGFLDTNP
AIREQTVKSMLLLAPKLNEANLNVELMK
 

Matching transcripts

SCYL1-001 - ENSP00000270176 [100%]
SCYL1-002 - ENSP00000408192 [100%]
SCYL1-003 - ENSP00000436993 [100%]
SCYL1-005 - ENSP00000432175 [100%]
SCYL1-006 - ENSP00000437254 [100%]
SCYL1-007 - ENSP00000433450 [100%]
SCYL1-014 - ENSP00000431635 [100%]
SCYL1-201 - ENSP00000279270 [100%]
 

Other gene match

 

ANTIBODY VALIDATION

         Immunohistochemistry

Image

 

Description

Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells and endothelial cells.
More information
 

Retrieval method

HIER pH6 

Antibody dilution

1:200 

Validation IH

Uncertain: One antibody yielding a staining pattern and there is no available gene/protein characterization data or the staining pattern is partly consistent with or contradicted by limited gene/protein characterization data 

         Western Blot

Image

 

Description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT-4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

89.6, 88.1, 86.4, 86.3, 86.2, 85.7, 78.7, 73.7 

Antibody dilution

1:250 

Validation WB

Supportive: Single band corresponding to the predicted size in kDa (+/-20%) 

         Protein array

Image

 

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:3000 

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen. 
 

ANTIGEN VIEW

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SCYL1-001
 
SCYL1-002
 
SCYL1-003
 
SCYL1-005
 
SCYL1-006
 
SCYL1-007
 
SCYL1-014
 
SCYL1-201