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SUBCELLULAR LOCATION

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Main location(s)

Nucleus but not nucleoli, Cytoplasm

Additional location(s)

 

Staining summary

Localized to the cytoplasm and nucleus.

Reliability (APE)

Low

Antibodies in assay

HPA028392, HPA028398, HPA028441
 

Antibody HPA028392

 

Antibody HPA028398

 

Antibody HPA028441

 

         Cell line A-431

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Location(s)

  Cytoplasm Nucleus but not nucleoli, Cytoplasm 

Staining characteristics

   Speckle 

Staining intensity

  Strong Moderate 

Validation

  
One/multiple location(s) supported by experimental gene/protein characterization data.
 
One/multiple location(s) supported by experimental gene/protein characterization data.
 

Comment

    

         Cell line U-2 OS

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Location(s)

Nucleus but not nucleoli Cytoplasm Nucleus but not nucleoli, Cytoplasm 

Staining characteristics

Spotty  Speckle 

Staining intensity

Moderate Strong Strong 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data.
 
One/multiple location(s) supported by experimental gene/protein characterization data.
 
One/multiple location(s) supported by experimental gene/protein characterization data.
 

Comment

   

         Cell line U-251 MG

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Location(s)

Nucleus but not nucleoli, Cytoplasm Cytoplasm Nucleus but not nucleoli, Cytoplasm 

Staining characteristics

Spotty  Speckle 

Staining intensity

Moderate Moderate Strong 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data.
 
One/multiple location(s) supported by experimental gene/protein characterization data.
 
One/multiple location(s) supported by experimental gene/protein characterization data.
 

Comment

   

         Antibody summary


Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus but not nucleoli.
 

Immunofluorescent staining of human cell line U-251 MG shows positivity in cytoplasm.
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus but not nucleoli & cytoplasm.
 

Summary

Staining of nucleus in U-251 MG and U-2 OS, with additional staining of cytoplasm in U-251 MG.   Staining of cytoplasm in all three cell lines.   Staining of nucleus and cytoplasm in all three cell lines.  

Validation

The subcellular location is supported by literature.
 
The subcellular location is supported by literature.
 
The subcellular location is supported by literature.
 

Dictionary

Cytoplasm
Nucleus but not nucleoli