ABL2

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA001866

 

Antibody CAB017106

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 SDIX
 

Product name

HPA001866 2327 

Host species

Rabbit Rabbit 

Clonality

pAb msAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand Affinity 

Released in version

3 4 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment Genetic immunization 

Length (aa)

136  

Antigen sequence

MAGVPEDGEQPGWPSPAKAAPVLPTTHNHKVPVLISPTLKHTPADVQLIG
TDSQGNKFKLLSEHQVTSSGDKDRPRRVKPKCAPPPPPVMRLLQHPSICS
DPTEEPTALTAGQSTSETQEGGKKAALGAVPISGKA
 
 

Matching transcripts

ABL2-001 - ENSP00000427562 [100%]
ABL2-002 - ENSP00000339209 [100%]
ABL2-005 - ENSP00000423578 [100%]
ABL2-006 - ENSP00000426831 [100%]
ABL2-008 - ENSP00000356595 [100%]
ABL2-009 - ENSP00000423413 [100%]
ABL2-010 - ENSP00000424697 [100%]
ABL2-201 - ENSP00000386152 [100%]
  

Other gene match

  

ANTIBODY VALIDATION

         Immunohistochemistry

Image

  

Description

Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in glial cells.
More information
 Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in neuronal and glial cells.
More information
 

Retrieval method

HIER pH6 HIER pH6 

Antibody dilution

1:20 1:350 

Validation IH

Uncertain: Two (or more) antibodies yielding similar or partly similar staining patterns which are partly consistent with or contradicted by limited gene/protein characterization data Uncertain: Two (or more) antibodies yielding similar or partly similar staining patterns which are partly consistent with or contradicted by limited gene/protein characterization data 

         Immunofluorescence

Image

  

Description

Immunofluorescent staining of human cell line A-431 shows positivity in nucleus but not nucleoli.
More information
 Immunofluorescent staining of human cell line U-251 MG shows positivity in nucleus but not nucleoli.
More information
 

Antibody dilution

1:56 1:87 

Validation IF

Uncertain: The subcellular location is partly supported by literature or no literature is available. Uncertain: The subcellular location is partly supported by literature or no literature is available. 

         Western Blot

Image

  

Description

Lane 1: Marker [kDa] 206, 113, 82, 49, 32, 26, 17.8
Lane 2: RT-4
Lane 3: U-251 MG
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil
More information
 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT-4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

128.3, 126.7, 126.2, 124.6, 117.9, 116.3, 115.8, 114.2 128.3, 126.7, 126.2, 124.6, 117.9, 116.3, 115.8, 114.2, 60.9 

Antibody dilution

1:250 1:500 

Validation WB

Uncertain: No bands detected Supportive: Band of predicted size in kDa (+/-20%) with additional bands present 

         Protein array

Image

  

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
  

Antibody dilution

1:3000  

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen.  
 

ANTIGEN VIEW

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ABL2-001
 
ABL2-002
 
ABL2-005
 
ABL2-006
 
ABL2-008
 
ABL2-009
 
ABL2-010
 
ABL2-201