TUFT1

ANTIBODY AND ANTIGEN INFORMATION

? »
 

Antibody HPA028112

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA028112 

Host species

Rabbit 

Clonality

pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

6 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment 

Length (aa)

99 

Antigen sequence

DCVAFEVTLSRYQREAEQSNVALQREEDRVEQKEAEVGELQRRLLGMETE
HQALLAKVREGEVALEELRSNNADCQAEREKAATLEKEVAGLREKIHHL
 

Matching transcripts

TUFT1-006 - ENSP00000357842 [100%]
TUFT1-008 - ENSP00000357841 [100%]
TUFT1-010 - ENSP00000376476 [100%]
TUFT1-201 - ENSP00000343781 [100%]
TUFT1-202 - ENSP00000437997 [100%]
 

Other gene match

 

ANTIBODY VALIDATION

         Immunohistochemistry

Image

 

Description

Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in glandular cells.
More information
 

Retrieval method

HIER pH6 

Antibody dilution

1:500 

Validation IH

Uncertain: One antibody yielding a staining pattern and there is no available gene/protein characterization data or the staining pattern is partly consistent with or contradicted by limited gene/protein characterization data 

         Western Blot

Image

 

Description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT-4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

46.4, 44.3, 41.5, 38.0, 37.9 

Antibody dilution

1:250 

Validation WB

Supportive: Band of predicted size in kDa (+/-20%) with additional bands present 

         Protein array

Image

 

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:3000 

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen. 
 

ANTIGEN VIEW

? »
 
 
 
TUFT1-006
 
TUFT1-008
 
TUFT1-010
 
TUFT1-201
 
TUFT1-202