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SUBCELLULAR LOCATION

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Main location(s)

Nucleus but not nucleoli, Cytoplasm

Additional location(s)

Plasma membrane, Golgi apparatus 

Staining summary

Staining of nucleus and cytoplasm in all three cell lines with additional staining of plasma membrane in A-431 and Golgi apparatus in U-2 OS.

Reliability (Single)

Antibodies in assay

HPA018142
 

Antibody HPA018142

 

         Cell line A-431

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Location(s)

Nucleus but not nucleoli, Plasma membrane, Cytoplasm 

Staining characteristics

 

Staining intensity

Moderate 

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

 

         Cell line U-2 OS

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Location(s)

Nucleus but not nucleoli, Cytoplasm, Golgi apparatus 

Staining characteristics

 

Staining intensity

Strong 

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

 

         Cell line U-251 MG

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Location(s)

Nucleus but not nucleoli, Cytoplasm 

Staining characteristics

 

Staining intensity

Strong 

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

 

         Antibody summary


Immunofluorescent staining of human cell line A-431 shows positivity in nucleus but not nucleoli, plasma membrane & cytoplasm.
 

Summary

Staining of nucleus and cytoplasm in all three cell lines with additional staining of plasma membrane in A-431 and Golgi apparatus in U-2 OS.  

Validation

The subcellular location is supported by literature.
 

Dictionary

Cytoplasm
Golgi apparatus
Nucleus but not nucleoli
Plasma membrane