Fields »

SUBCELLULAR LOCATION

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Main location(s)

Nucleus

Additional location(s)

 

Staining summary

Localized to nucleus.

Reliability (APE)

High

Antibodies in assay

HPA001759, CAB004318
 

Antibody HPA001759

 

Antibody CAB004318

 

         Cell line A-431

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Location(s)

Nucleus Nucleus 

Staining characteristics

Spotty  

Staining intensity

Moderate Weak 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 

Comment

  

         Cell line U-2 OS

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Location(s)

Nucleus Nucleus but not nucleoli 

Staining characteristics

Granular  

Staining intensity

Weak Weak 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 

Comment

  

         Cell line U-251 MG

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Location(s)

Nucleus Nucleus but not nucleoli 

Staining characteristics

Spotty  

Staining intensity

Weak Weak 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line A-431 shows positivity in nuclei.
 

Immunofluorescent staining of human cell line U-251 MG shows positivity in nucleus but not nucleoli.
 

Summary

Staining of nuclei in all three cell lines with additional stringer staining of spots in nuclei in U-251MG and A-431.   Staining of nucleus in all three cell lines.  

Validation

The subcellular location is supported by literature.
 
The subcellular location is supported by literature.
 

Dictionary

Nucleus