Fields »

SUBCELLULAR LOCATION

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Main location(s)

Nucleus, Plasma membrane, Cytoplasm

Additional location(s)

 

Staining summary

Staining of plasma membrane, cytoplasm and nucleus in all three cell lines

Reliability (Single)

Antibodies in assay

HPA028220
 

Antibody HPA028220

 

         Cell line A-431

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Location(s)

Nucleus, Plasma membrane, Cytoplasm 

Staining characteristics

Spotty 

Staining intensity

Moderate 

Validation

Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 

Comment

 

         Cell line U-2 OS

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Location(s)

Nucleus, Plasma membrane, Cytoplasm 

Staining characteristics

Spotty 

Staining intensity

Moderate 

Validation

Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 

Comment

 

         Cell line U-251 MG

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Location(s)

Nucleus but not nucleoli, Plasma membrane, Cytoplasm 

Staining characteristics

Spotty 

Staining intensity

Moderate 

Validation

Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 

Comment

 

         Antibody summary


Immunofluorescent staining of human cell line U-251MG shows positivity in nucleus but not nucleoli, plasma membrane & cytoplasm.
 

Summary

Staining of plasma membrane, cytoplasm and nucleus in all three cell lines  

Validation

The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Cytoplasm
Nucleus
Plasma membrane