PDIA6

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA034652

 

Antibody HPA034653

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA034652 HPA034653 

Host species

Rabbit Rabbit 

Clonality

pAb pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

12 6 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment Recombinant protein fragment 

Length (aa)

93 98 

Antigen sequence

YGIRGFPTIKIFQKGESPVDYDGGRTRSDIVSRALDLFSDNAPPPELLEI
INEDIAKRTCEEHQLCVVAVLPHILDTGAAGRNSYLEVLLKLA
 
GWLWTEAGAQSELETALGIGGFGYPAMAAINARKMKFALLKGSFSEQGIN
EFLRELSFGRGSTAPVGGGAFPTIVEREPWDGRDGELPVEDDIDLSDV
 

Matching transcripts

PDIA6-001 - ENSP00000272227 [100%]
PDIA6-002 - ENSP00000385385 [100%]
PDIA6-003 - ENSP00000384459 [100%]
PDIA6-201 - ENSP00000371024 [100%]
PDIA6-202 - ENSP00000438778 [100%]
 PDIA6-001 - ENSP00000272227 [100%]
PDIA6-002 - ENSP00000385385 [100%]
PDIA6-003 - ENSP00000384459 [100%]
PDIA6-201 - ENSP00000371024 [100%]
PDIA6-202 - ENSP00000438778 [100%]
 

Other gene match

  

ANTIBODY VALIDATION

         Immunofluorescence

Image

  

Description

Immunofluorescent staining of human cell line U-2 OS shows positivity in endoplasmic reticulum.
More information
  

Antibody dilution

1:100  

Validation IF

Uncertain: The subcellular location is partly supported by literature or no literature is available.  

         Western Blot

Image

  

Description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT-4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT-4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

53.9, 53.3, 48.6, 48.1, 47.8 53.9, 53.3, 48.6, 48.1, 47.8 

Antibody dilution

1:250 1:250 

Validation WB

Supportive: Band of predicted size in kDa (+/-20%) with additional bands present Supportive: Single band corresponding to the predicted size in kDa (+/-20%) 

         Protein array

Image

  

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:3000 1:3000 

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen. Supportive: Pass with single peak corresponding to interaction only with its own antigen. 

         Immunohistochemistry

Image

  

Description

 Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.
More information
 

Retrieval method

 HIER pH6 

Antibody dilution

 1:50 

Validation IH

 Supportive: Two (or more) antibodies yielding similar staining patterns which are partly consistent with gene/protein characterization data or consistent with limited gene/protein characterization data 
 

ANTIGEN VIEW

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PDIA6-001
 
PDIA6-002
 
PDIA6-003
 
PDIA6-201
 
PDIA6-202