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SUBCELLULAR LOCATION

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Main location(s)

Mitochondria

Additional location(s)

 

Staining summary

Mainly localized to mitochondria.

Reliability (APE)

High

Antibodies in assay

HPA019714, HPA019716, HPA019848, HPA026720
 

Antibody HPA019714

 

Antibody HPA019716

 

Antibody HPA019848

 

Antibody HPA026720

 

         Cell line A-431

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Location(s)

Mitochondria Mitochondria Nucleus but not nucleoli, Plasma membrane, Mitochondria Mitochondria 

Staining characteristics

    

Staining intensity

Strong Moderate Moderate Strong 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 
Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 

Comment

    

         Cell line U-2 OS

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Location(s)

Mitochondria Mitochondria Plasma membrane, Mitochondria Mitochondria 

Staining characteristics

    

Staining intensity

Strong Strong Strong Strong 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 
Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 

Comment

    

         Cell line U-251 MG

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Location(s)

 Mitochondria Plasma membrane, Mitochondria Mitochondria 

Staining characteristics

    

Staining intensity

Negative Strong Strong Strong 

Validation

No staining.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 
Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 

Comment

    

         Antibody summary


Immunofluorescent staining of human cell line U-2 OS shows positivity in mitochondria.
 

Immunofluorescent staining of human cell line U-251MG shows positivity in mitochondria.
 

Immunofluorescent staining of human cell line A-431 shows positivity in nucleus but not nucleoli, plasma membrane & mitochondria.
 

Immunofluorescent staining of human cell line U-251MG shows positivity in mitochondria.
 

Summary

Staining of mitochondria in A-431 and U-2 OS.   Staining of mitochondria in all three cell lines.   Staining of mitochondria and plasma membrane in all three cell lines with additional staining of nuclei in A-431.   Staining of mitochondria in a fraction of the cells in all three cell lines.  

Validation

The subcellular location is supported by literature.
 
The subcellular location is supported by literature.
 
The subcellular location is supported by literature.
 
The subcellular location is supported by literature.
 

Dictionary

Mitochondria