AGBL3

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA020397

 

Antibody HPA024150

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA020397 HPA024150 

Host species

Rabbit Rabbit 

Clonality

pAb pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

5 5 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment Recombinant protein fragment 

Length (aa)

83 90 

Antigen sequence

CPEPVYIPTGLETQPLYPDSKEATVVYLAEDAYKEPCFVYSRVGGNRTPL
KQPVDYRDNTLMFEARFESGNLQKVVKVAEYEY
 
DYSDRTISDEDESDEDMFMKFVSEDLHRCALLTADSFGDPFFPRTTQILL
EYQLGRWVPRLREPRDLYGVSSSGPLSPTRWPYHCEVIDE
 

Matching transcripts

AGBL3-001 - ENSP00000275763 [99%]
AGBL3-006 - ENSP00000401220 [99%]
AGBL3-007 - ENSP00000388275 [99%]
AGBL3-202 - ENSP00000395969 [99%]
 AGBL3-001 - ENSP00000275763 [100%]
AGBL3-006 - ENSP00000401220 [100%]
AGBL3-007 - ENSP00000388275 [100%]
AGBL3-201 - ENSP00000352343 [100%]
AGBL3-202 - ENSP00000395969 [80%]
 

Other gene match

  

ANTIBODY VALIDATION

         Immunohistochemistry

Image

  

Description

Immunohistochemical staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.
More information
 Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.
More information
 

Retrieval method

HIER pH6 HIER pH6 

Antibody dilution

1:25 1:250 

Literature conformity

Partly consistent with extensive gene/protein characterization data No avaliable characterization data 

RNA consistency

Not done Not done 

         Immunofluorescence

Image

  

Description

Immunofluorescent staining of human cell line U-251 MG shows positivity in cytoplasm.
More information
 Immunofluorescent staining of human cell line U-2 OS shows positivity in mitochondria.
More information
 

Antibody dilution

1:50 1:100 

Validation IF

Uncertain: The subcellular location is partly supported by literature or no literature is available. Uncertain: The subcellular location is partly supported by literature or no literature is available. 

         Western Blot

Image

  

Description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
  

Target mass (kDa)

112.9, 107.0, 82.1, 72.6 112.9, 107.0, 82.1, 72.6, 20.4 

Antibody dilution

1:250 1:250 

Validation WB

Uncertain: No bands detected Non-supportive: Weak band of predicted size but with additional bands of higher intensity also present 

         Protein array

Image

  

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:500 1:3000 

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen. Supportive: Pass with single peak corresponding to interaction only with its own antigen. 
 

ANTIGEN VIEW

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AGBL3-001
 
AGBL3-006
 
AGBL3-007
 
AGBL3-201
 
AGBL3-202