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SUBCELLULAR LOCATION

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Main location(s)

Nucleus but not nucleoli, Cytoplasm, Mitochondria

Additional location(s)

 

Staining summary

Localized to the nucleus, cytoplasm and mitochondria.

Reliability (APE)

Very low

Antibodies in assay

HPA020397, HPA024150
 

Antibody HPA020397

 

Antibody HPA024150

 

         Cell line A-431

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Location(s)

Cytoplasm Nucleus but not nucleoli, Mitochondria 

Staining characteristics

Granular Speckle 

Staining intensity

Weak Weak 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 

Comment

  

         Cell line U-2 OS

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Location(s)

  Mitochondria 

Staining characteristics

   

Staining intensity

  Strong 

Validation

  
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 

Comment

   

         Cell line U-251 MG

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Location(s)

Cytoplasm Nucleus but not nucleoli 

Staining characteristics

 Speckle 

Staining intensity

Moderate Weak 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line U-251MG shows positivity in cytoplasm.
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in mitochondria.
 

Summary

Staining of cytoplasm in U-251 MG and A-431.   Staining of nucleus in U-251 MG. Staining of mitochondria in U-2 OS. Staining of both nucleus and mitochondria in A-431.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Cytoplasm
Mitochondria
Nucleus but not nucleoli