The normal tissue page shows antibody staining and, when available, knowledge-based annotated protein expression in 46 human tissues. The assay and annotation is described here.
This page starts with a summary. The summary data can be based on one or more antibodies (reported by the "Antibodies in assay"-field). If the data is based on one single antibody, the IH validation score for that antibody is reported in the "Reliability (Single)"-field (color-coding described here). If the data is based on more than one antibody, the reliability score for the data is reported as "Reliability (APE)" (color-coding described here).
The tissues and cell types can be ordered by organ, cell type or alphabetically.
For each tissue and cell type, the antibody staining is given with the yellow-scale color-coding (described by the scale in the box to the right). Each available antibody is listed in a separate column and the antibody identifier is available at the bottom of the tissue list. The images and annotations can be accessed by clicking on the tissue name.
When available, a knowledge-based annotated protein expression (APE) is given to the right of the antibody staining values. The level of expression is given by a blue-scale color-coding (described by the scale in the box to the right).
At the very bottom of this page, a summary of the staining from each antibody is given, together with a representative image and the immunohistochemistry validation score for the antibody.
Nuclear expression in gastrointestinal tract, squamous epithelia and lymphoid tissues. Weaker expression in several other tissues.
Caution: Based on antibodies targeting proteins from multiple genes.
Immunohistochemical staining of human appendix shows dot like positivity in nuclei of some lymphoid cells.
Immunohistochemical staining of human bone marrow shows distinct cytoplasmic positivity in subsets of hematopoietic cells.
Immunohistochemical staining of human duodenum shows strong nuclear positivity in glandular cells.
Summary
Nuclear staining with a dot-like pattern was observed in proliferating cells, mainly in lymphoid tissues and glandular epithelia. Remaning normal cells were in general negative.
Bone marrow poetic cells, a sub-set of cells in spleen and lymph node, macrophages and plasma cells showed strong nuclear positivity. Remaining normal cells were negative.
Gastrointestinal tract, squamous epithelium, female genitalia and lymphoid tissues showed moderate to strong nuclear positivity. Remaining normal cells were weakly stained or negative.
Validation
The multi-targeting antibody yielding a staining pattern partly consistent with gene/protein characterization data or consistent with limited gene/protein characterization data for all of the genes
The multi-targeting antibody yielding a staining pattern partly consistent with gene/protein characterization data or consistent with limited gene/protein characterization data for all of the genes
One antibody yielding a staining pattern which is partly consistent with gene/protein characterization data or consistent with limited gene/protein characterization data
