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SUBCELLULAR LOCATION

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Main location(s)

Nucleus, Nucleoli, Nucleus but not nucleoli

Additional location(s)

Cytoplasm, Golgi apparatus 

Staining summary

Mainly localized to nucleus and nucleoli but also to cytoplasm and Golgi apparatus.

Reliability (APE)

Very low

Antibodies in assay

HPA000568, HPA001165, HPA002111
 

Antibody HPA000568

 

Antibody HPA001165

 

Antibody HPA002111

 

         Cell line A-431

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Location(s)

Nucleoli Cytoplasm Nucleus but not nucleoli, Golgi apparatus 

Staining characteristics

   

Staining intensity

Moderate Moderate Weak 

Validation

The multi-targeting antibody yielding a staining pattern with no available gene/protein characterization data.
 
The multi-targeting antibody yielding a staining pattern with no available gene/protein characterization data.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 

Comment

   

         Cell line U-2 OS

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Location(s)

Nucleoli Cytoplasm Nucleus but not nucleoli, Golgi apparatus 

Staining characteristics

  Granular 

Staining intensity

Weak Moderate Weak 

Validation

The multi-targeting antibody yielding a staining pattern with no available gene/protein characterization data.
 
The multi-targeting antibody yielding a staining pattern with no available gene/protein characterization data.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 

Comment

   

         Cell line U-251 MG

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Location(s)

Nucleoli Cytoplasm Nucleus but not nucleoli, Golgi apparatus 

Staining characteristics

  Granular 

Staining intensity

Weak Moderate Weak 

Validation

The multi-targeting antibody yielding a staining pattern with no available gene/protein characterization data.
 
The multi-targeting antibody yielding a staining pattern with no available gene/protein characterization data.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 

Comment

   

         Antibody summary


Immunofluorescent staining of human cell line U-251MG shows positivity in nucleoli.
 

Immunofluorescent staining of human cell line U-251 MG shows positivity in cytoplasm.
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus but not nucleoli & golgi apparatus.
 

Summary

Staining of nucleoli in a fraction of the cells in all three cell lines.   Staining of the cytoplasm in all three cell lines.   Staining of nucleus and the Golgi apparatus in all three cell lines.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Cytoplasm
Golgi apparatus
Nucleoli
Nucleus
Nucleus but not nucleoli