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SUBCELLULAR LOCATION

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Main location(s)

Plasma membrane

Additional location(s)

Nucleus, Nucleoli 

Staining summary

Mainly localized to the plasma membrane.

Reliability (APE)

High

Antibodies in assay

HPA000263, HPA011135, HPA011227, CAB047338
 

Antibody HPA000263

 

Antibody HPA011135

 

Antibody HPA011227

 

Antibody CAB047338

 

         Cell line A-431

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Location(s)

  Plasma membrane, Cytoplasm Nucleus, Plasma membrane Plasma membrane, Cytoplasm 

Staining characteristics

  Granular Granular  

Staining intensity

  Moderate Weak Weak 

Validation

  
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 
Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 

Comment

     

         Cell line U-2 OS

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Location(s)

 Plasma membrane Nucleus, Nucleoli, Plasma membrane Plasma membrane, Cytoplasm 

Staining characteristics

    

Staining intensity

Negative Moderate Moderate Weak 

Validation

The multi-targeting antibody not yielding a staining pattern.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 
Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 

Comment

    

         Cell line U-251 MG

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Location(s)

 Plasma membrane Nucleus, Nucleoli, Plasma membrane Plasma membrane, Cytoplasm 

Staining characteristics

    

Staining intensity

Negative Strong Moderate Moderate 

Validation

The multi-targeting antibody not yielding a staining pattern.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 
Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 

Comment

    

         Antibody summary


Immunofluorescent staining of human cell line U-251MG shows no positivity.
 

Immunofluorescent staining of human cell line U-251MG shows positivity in plasma membrane.
 

Immunofluorescent staining of human cell line U-251MG shows positivity in nucleus, nucleoli & plasma membrane.
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in plasma membrane & cytoplasm.
 

Summary

No staining was observed in any of the three cell lines.   Staining of plasma membrane in all three cell lines with additional staining of cytoplasm in A-431.   Staining of plasma membrane and nucleus in all three cell lines with additional staining of nucleoli in U-251 MG and U-2 OS.   Staining of cytoplasm and plasma membrane in all three cell lines.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is supported by literature.
 
The subcellular location is supported by literature.
 
The subcellular location is supported by literature.
 

Dictionary

Nucleoli
Nucleus
Plasma membrane