Fields »

SUBCELLULAR LOCATION

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Main location(s)

Nucleus

Additional location(s)

Cytoplasm 

Staining summary

Localized to the nucleus and cytoplasm.

Reliability (APE)

Very low

Antibodies in assay

HPA005949, HPA020046
 

Antibody HPA005949

 

Antibody HPA020046

 

         Cell line A-431

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Location(s)

Nucleus Nucleoli 

Staining characteristics

  

Staining intensity

Moderate Moderate 

Validation

Location not consistent with experimental gene/protein characterization data and showing dissimilar staining to ≥1 other antibody.
 
Location not consistent with experimental gene/protein characterization data and showing dissimilar staining to ≥1 other antibody.
 

Comment

  

         Cell line U-2 OS

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Location(s)

Cytoplasm Nucleoli 

Staining characteristics

Granular  

Staining intensity

Moderate Strong 

Validation

Location not consistent with experimental gene/protein characterization data and showing dissimilar staining to ≥1 other antibody.
 
Location not consistent with experimental gene/protein characterization data and showing dissimilar staining to ≥1 other antibody.
 

Comment

  

         Cell line U-251 MG

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Location(s)

Nucleus Nucleoli 

Staining characteristics

 Cluster 

Staining intensity

Moderate Strong 

Validation

Location not consistent with experimental gene/protein characterization data and showing dissimilar staining to ≥1 other antibody.
 
Location not consistent with experimental gene/protein characterization data and showing dissimilar staining to ≥1 other antibody.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line U-2 OS shows positivity in cytoplasm.
 

Immunofluorescent staining of human cell line A-431 shows positivity in nucleoli.
 

Summary

Staining of nucleus in U-251MG and A-431. Staining of cytoplasm in U-2 OS.   Staining of nucleoli in all three cell lines.  

Validation

The subcellular location is not consistent with literature.
 
The subcellular location is not consistent with literature.
 

Dictionary

Cytoplasm
Nucleus