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SUBCELLULAR LOCATION

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Main location(s)

Nucleoli, Golgi apparatus, Vesicles

Additional location(s)

Cytoplasm, Mitochondria 

Staining summary

Staining of Golgi and vesicles in U-251 MG, nucleoli and mitochondria in A-431 and of nucleoli, vesicles and cytoplasm in U-2 OS.

Reliability (Single)

Antibodies in assay

HPA005647
 

Antibody HPA005647

 

         Cell line A-431

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Location(s)

Nucleoli, Mitochondria 

Staining characteristics

Cluster 

Staining intensity

Strong 

Validation

One/multiple location(s) in cytoplasm (e.g. golgi, mitochondria) supported by experimental evidence for cytoplasmic localization.
 

Comment

 

         Cell line U-2 OS

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Location(s)

Nucleoli, Cytoplasm, Vesicles 

Staining characteristics

 

Staining intensity

Moderate 

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

 

         Cell line U-251 MG

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Location(s)

Golgi apparatus, Vesicles 

Staining characteristics

 

Staining intensity

Strong 

Validation

One/multiple location(s) in cytoplasm (e.g. golgi, mitochondria) supported by experimental evidence for cytoplasmic localization.
 

Comment

 

         Antibody summary


Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleoli, cytoplasm & vesicles.
 

Summary

Staining of Golgi and vesicles in U-251 MG, nucleoli and mitochondria in A-431 and of nucleoli, vesicles and cytoplasm in U-2 OS.  

Validation

The subcellular location is supported by literature.
 

Dictionary

Cytoplasm
Golgi apparatus
Mitochondria
Nucleoli
Endosome