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SUBCELLULAR LOCATION

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Main location(s)

Cytoplasm, Cytoskeleton (Intermediate filaments)

Additional location(s)

 

Staining summary

Localized to the cytoplasm or to intermediate filaments.

Reliability (APE)

Very low

Antibodies in assay

HPA006316, CAB017784
 

Antibody HPA006316

 

Antibody CAB017784

 

         Cell line A-431

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Location(s)

Cytoskeleton (Intermediate filaments) Cytoplasm 

Staining characteristics

  

Staining intensity

Strong Moderate 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 

Comment

  

         Cell line U-2 OS

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Location(s)

  Cytoplasm 

Staining characteristics

   

Staining intensity

  Weak 

Validation

  
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 

Comment

   

         Cell line U-251 MG

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Location(s)

  Cytoplasm 

Staining characteristics

   

Staining intensity

  Moderate 

Validation

  
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 

Comment

   

         Antibody summary


Immunofluorescent staining of human cell line A-431 shows positivity in cytoskeleton (intermediate filaments).
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in cytoplasm.
 

Summary

Staining of cytoskeleton (intermediate filaments) in A-431.   Staining of cytoplasm in all three cell lines.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Cytoplasm
Intermediate filaments