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SUBCELLULAR LOCATION

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Main location(s)

Plasma membrane, Cytoplasm

Additional location(s)

 

Staining summary

Localized to plasma membrane and cytoplasm.

Reliability (APE)

Medium

Antibodies in assay

HPA000400, HPA016438
 

Antibody HPA000400

 

Antibody HPA016438

 

         Cell line A-431

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Location(s)

Plasma membrane, Cytoplasm Plasma membrane, Cytoplasm 

Staining characteristics

  

Staining intensity

Moderate Strong 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 
One/multiple location(s) with no available experimental gene/protein characterization data.
 

Comment

  

         Cell line U-2 OS

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Location(s)

Plasma membrane, Cytoplasm, Focal Adhesions Plasma membrane, Cytoplasm 

Staining characteristics

  

Staining intensity

Moderate Moderate 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 
One/multiple location(s) with no available experimental gene/protein characterization data.
 

Comment

  

         Cell line U-251 MG

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Location(s)

Plasma membrane, Cytoplasm Nucleus but not nucleoli, Cytoplasm 

Staining characteristics

  

Staining intensity

Weak Moderate 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 
One/multiple location(s) with no available experimental gene/protein characterization data.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line U-2 OS shows positivity in plasma membrane, cytoskeleton (actin filaments) & focal adhesions.
 

Immunofluorescent staining of human cell line A-431 shows positivity in plasma membrane & cytoplasm.
 

Summary

Staining of plasma membrnae/cytoplasm in all three cell lines. Additional staining of nuclei in U-251 MG and focal adhesion sites and actin filaments in U-2 OS.   Staining of plasma membrane/cytoplasm in A-431 and U-2 OS. Staining of nuclei but not nucleoli and cytoplasm in U-251 MG.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Cytoplasm
Plasma membrane