Fields »

SUBCELLULAR LOCATION

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Main location(s)

Cytoskeleton (Intermediate filaments), Cytoskeleton (Microtubules)

Additional location(s)

Cytoplasm 

Staining summary

Staining of cytoskeleton (intermediate filaments) in U-251 MG and cytoplasm and cytoskeleton (microtubules) in U-2 OS.

Reliability (Single)

Antibodies in assay

HPA024702
 

Antibody HPA024702

 

         Cell line A-431

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Location(s)

 

Staining characteristics

 

Staining intensity

Negative 

Validation

No staining.
 

Comment

 

         Cell line U-2 OS

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Location(s)

Cytoplasm, Cytoskeleton (Microtubules) 

Staining characteristics

Granular 

Staining intensity

Moderate 

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

 

         Cell line U-251 MG

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Location(s)

Cytoskeleton (Intermediate filaments) 

Staining characteristics

Granular 

Staining intensity

Weak 

Validation

One/multiple location(s) in cytoplasm (e.g. golgi, mitochondria) supported by experimental evidence for cytoplasmic localization.
 

Comment

 

         Antibody summary


Immunofluorescent staining of human cell line U-2 OS shows positivity in cytoplasm & cytoskeleton (microtubules).
 

Summary

Staining of cytoskeleton (intermediate filaments) in U-251 MG and cytoplasm and cytoskeleton (microtubules) in U-2 OS.  

Validation

The subcellular location is supported by literature.
 

Dictionary

Cytoplasm
Intermediate filaments
Microtubules