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SUBCELLULAR LOCATION

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Main location(s)

Nucleoli, Cytoplasm

Additional location(s)

 

Staining summary

Localized to the cytoplasm and nucleoli.

Reliability (APE)

High

Antibodies in assay

HPA020600, HPA021088, HPA029522
 

Antibody HPA020600

 

Antibody HPA021088

 

Antibody HPA029522

 

         Cell line A-431

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Location(s)

Nucleoli, Cytoplasm Nucleoli, Cytoplasm Nucleus, Nucleoli, Cytoplasm 

Staining characteristics

  Speckle 

Staining intensity

Strong Moderate Moderate 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but supported by ≥1 other antibody.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but supported by ≥1 other antibody.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but supported by ≥1 other antibody.
 

Comment

   

         Cell line U-2 OS

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Location(s)

Nucleoli, Cytoplasm Nucleoli, Cytoplasm Nucleoli, Cytoplasm 

Staining characteristics

   

Staining intensity

Moderate Moderate Strong 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but supported by ≥1 other antibody.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but supported by ≥1 other antibody.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but supported by ≥1 other antibody.
 

Comment

   

         Cell line U-251 MG

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Location(s)

Nucleoli, Cytoplasm Nucleoli, Cytoplasm Nucleoli, Cytoplasm 

Staining characteristics

   

Staining intensity

Moderate Moderate Strong 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but supported by ≥1 other antibody.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but supported by ≥1 other antibody.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but supported by ≥1 other antibody.
 

Comment

   

         Antibody summary


Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleoli & cytoplasm.
 

Immunofluorescent staining of human cell line A-431 shows positivity in nucleoli & cytoplasm.
 

Immunofluorescent staining of human cell line U-251MG shows positivity in nucleoli & cytoplasm.
 

Summary

Staining of nucleoli and cytoplasm in all three cell lines.   Staining of nucleoli and cytoplasm in all three cell lines.   Staining of nucleoli and cytoplasm in all three cell lines.  

Validation

The subcellular location is supported by literature.
 
The subcellular location is supported by literature.
 
The subcellular location is supported by literature.
 

Dictionary

Cytoplasm
Nucleoli