SH3GLB2

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA021438

 

Antibody HPA024734

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA021438 HPA024734 

Host species

Rabbit Rabbit 

Clonality

pAb pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

5 5 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment Recombinant protein fragment 

Length (aa)

82 91 

Antigen sequence

RRLDLDACKARLKKAKAAEAKATTVPDFQETRPRNYILSASASALWNDEV
DKAEQELRVAQTEFDRQAEVTRLLLEGISSTH
 
HDFVKSQTTYYAQCYRHMLDLQKQLGRFPGTFVGTTEPASPPLSSTSPTT
AAATMPVVPSVASLAPPGEASLCLEEVAPPASGTRKARVLY
 

Matching transcripts

SH3GLB2-001 - ENSP00000361645 [100%]
SH3GLB2-002 - ENSP00000361640 [100%]
SH3GLB2-011 - ENSP00000402566 [100%]
SH3GLB2-008 - ENSP00000361634 [95%]
 SH3GLB2-001 - ENSP00000361645 [99%]
SH3GLB2-002 - ENSP00000361640 [99%]
SH3GLB2-012 - ENSP00000388282 [99%]
SH3GLB2-008 - ENSP00000361634 [93%]
SH3GLB2-011 - ENSP00000402566 [93%]
 

Other gene match

  

ANTIBODY VALIDATION

         Immunohistochemistry

Image

  

Description

Immunohistochemical staining of human rectum shows moderate positivity in glandular cells.
More information
 Immunohistochemical staining of human urinary bladder shows strong cytoplasmic positivity in urothelial cells.
More information
 

Retrieval method

HIER pH6 HIER pH6 

Antibody dilution

1:750 1:225 

Validation IH

Uncertain: Two (or more) antibodies yielding partly similar staining patterns which are partly consistent with gene/protein characterization data or consistent with limited gene/protein characterization data Uncertain: Two (or more) antibodies yielding partly similar staining patterns which are partly consistent with gene/protein characterization data or consistent with limited gene/protein characterization data 

         Western Blot

Image

  

Description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT-4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: Negative control (vector only transfected HEK293T lysate)
Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY402767)
More information
 

Target mass (kDa)

44.8, 44.4, 44.0 44.8, 44.4, 44.0, 41.7 

Antibody dilution

1:250 1:250 

Validation WB

Supportive: Band of predicted size in kDa (+/-20%) with additional bands present Supportive: Band of predicted size in kDa (+/-20%) with additional bands present 

         Protein array

Image

  

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:3000 1:3000 

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen. Supportive: Pass with single peak corresponding to interaction only with its own antigen. 
 

ANTIGEN VIEW

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SH3GLB2-001
 
SH3GLB2-002
 
SH3GLB2-008
 
SH3GLB2-011
 
SH3GLB2-012