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SUBCELLULAR LOCATION

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Main location(s)

Nucleus, Plasma membrane

Additional location(s)

Nucleoli, Cytoplasm 

Staining summary

Staining of nucleus and plasma membrane in all three cell lines with additional staining of centrosome in U-251 MG and A-431.

Reliability (Single)

Antibodies in assay

HPA027933
 

Antibody HPA027933

 

         Cell line A-431

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Location(s)

Nucleus, Nucleoli, Plasma membrane, Centrosome 

Staining characteristics

 

Staining intensity

Moderate 

Validation

Location not consistent with experimental gene/protein characterization data.
 

Comment

 

         Cell line U-2 OS

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Location(s)

Nucleus but not nucleoli, Plasma membrane, Cytoplasm 

Staining characteristics

 

Staining intensity

Weak 

Validation

Location not consistent with experimental gene/protein characterization data.
 

Comment

 

         Cell line U-251 MG

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Location(s)

Nucleus but not nucleoli, Plasma membrane, Centrosome 

Staining characteristics

Spotty 

Staining intensity

Moderate 

Validation

Location not consistent with experimental gene/protein characterization data.
 

Comment

 

         Antibody summary


Immunofluorescent staining of human cell line A-431 shows positivity in nucleus, nucleoli, plasma membrane & centrosome.
 

Summary

Staining of nucleus and plasma membrane in all three cell lines with additional staining of centrosome in U-251 MG and A-431.  

Validation

The subcellular location is not consistent with literature.
 

Dictionary

Cytoplasm
Nucleoli
Nucleus
Plasma membrane