Fields »

SUBCELLULAR LOCATION

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Main location(s)

Nucleus but not nucleoli

Additional location(s)

Plasma membrane 

Staining summary

Staining of plasma membrane and nucleus in all three cell lines.

Reliability (Single)

Antibodies in assay

HPA006026
 

Antibody HPA006026

 

         Cell line A-431

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Location(s)

Nucleus but not nucleoli, Plasma membrane 

Staining characteristics

Spotty 

Staining intensity

Weak 

Validation

Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 

Comment

 

         Cell line U-2 OS

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Location(s)

Nucleus but not nucleoli, Plasma membrane 

Staining characteristics

Spotty 

Staining intensity

Moderate 

Validation

Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 

Comment

 

         Cell line U-251 MG

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Location(s)

Nucleus but not nucleoli, Plasma membrane, Cell Junctions 

Staining characteristics

Spotty 

Staining intensity

Weak 

Validation

Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 

Comment

 

         Antibody summary


Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus but not nucleoli & plasma membrane.
 

Summary

Staining of plasma membrane and nucleus in all three cell lines.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Nucleus but not nucleoli
Plasma membrane