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SUBCELLULAR LOCATION

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Main location(s)

Plasma membrane, Golgi apparatus, Vesicles

Additional location(s)

 

Staining summary

Staining of Golgi in all three cell lines, additional staining of vesicles in U-2 OS and plasma membrane in A-431.

Reliability (Single)

Antibodies in assay

HPA002997
 

Antibody HPA002997

 

         Cell line A-431

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Location(s)

Plasma membrane, Golgi apparatus 

Staining characteristics

 

Staining intensity

Strong 

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

 

         Cell line U-2 OS

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Location(s)

Golgi apparatus, Vesicles 

Staining characteristics

 

Staining intensity

Strong 

Validation

Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 

Comment

 

         Cell line U-251 MG

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Location(s)

Golgi apparatus 

Staining characteristics

 

Staining intensity

Strong 

Validation

Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 

Comment

 

         Antibody summary


Immunofluorescent staining of human cell line A-431 shows positivity in plasma membrane & golgi apparatus.
 

Summary

Staining of Golgi in all three cell lines, additional staining of vesicles in U-2 OS and plasma membrane in A-431.  

Validation

The subcellular location is supported by literature.
 

Dictionary

Golgi apparatus
Plasma membrane
Endosome