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SUBCELLULAR LOCATION

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Main location(s)

Nucleus but not nucleoli, Cytoplasm

Additional location(s)

Nuclear membrane 

Staining summary

Localized to the cytoplasm.

Reliability (APE)

Very low

Antibodies in assay

HPA015762, CAB022698
 

Antibody HPA015762

 

Antibody CAB022698

 

         Cell line A-431

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Location(s)

 Cytoplasm 

Staining characteristics

  

Staining intensity

Negative Moderate 

Validation

No staining.
 
Location not consistent with experimental gene/protein characterization data.
 

Comment

  

         Cell line U-2 OS

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Location(s)

 Cytoplasm 

Staining characteristics

  

Staining intensity

Negative Moderate 

Validation

No staining.
 
Location not consistent with experimental gene/protein characterization data.
 

Comment

  

         Cell line U-251 MG

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Location(s)

 Nuclear membrane, Cytoplasm 

Staining characteristics

  

Staining intensity

Negative Moderate 

Validation

No staining.
 
Location not consistent with experimental gene/protein characterization data.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line U-2 OS shows no positivity.
 

Immunofluorescent staining of human cell line U-251 MG shows positivity in nuclear membrane & cytoplasm.
 

Summary

No staining was observed in any of the three cell lines.   Staining of cytoplasm in all three cell lines with additional staining of nuclear membrane in U-251 MG.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is not consistent with literature.
 

Dictionary

Cytoplasm
Nuclear membrane
Nucleus but not nucleoli