The normal tissue page shows antibody staining and, when available, knowledge-based annotated protein expression in 46 human tissues. The assay and annotation is described here.
This page starts with a summary. The summary data can be based on one or more antibodies (reported by the "Antibodies in assay"-field). If the data is based on one single antibody, the IH validation score for that antibody is reported in the "Reliability (Single)"-field (color-coding described here). If the data is based on more than one antibody, the reliability score for the data is reported as "Reliability (APE)" (color-coding described here).
The tissues and cell types can be ordered by organ, cell type or alphabetically.
For each tissue and cell type, the antibody staining is given with the yellow-scale color-coding (described by the scale in the box to the right). Each available antibody is listed in a separate column and the antibody identifier is available at the bottom of the tissue list. The images and annotations can be accessed by clicking on the tissue name.
When available, a knowledge-based annotated protein expression (APE) is given to the right of the antibody staining values. The level of expression is given by a blue-scale color-coding (described by the scale in the box to the right).
At the very bottom of this page, a summary of the staining from each antibody is given, together with a representative image and the immunohistochemistry validation score for the antibody.
Immunohistochemical staining of human lymph node shows secreted positivity.
Immunohistochemical staining of human lymph node shows distinct secreted positivity in a subset of reaction center cells.
Immunohistochemical staining of human tonsil shows distinct secreted positivity in subsets of lymphoid cells.
Summary
Macrophages were strongly stained. The gastrointestinal tract, bladder and squamous epithelia displayed moderate staining of cytoplasm and weaker staining of the nuclei. Subsets of cells in renal tubules showed distinct staining. Remaining normal tissues were in general weakly stained or negative.
Nuclear and cytoplasmic stining with moderate to strong intensity was observed in the gastrointestinal tract, lymphoid cells, squamous and respiratory epithelia as well as subsets of cells in renal tubules. Macrophages showed distinct staining. Muscle, endocrine cells and most cells in the CNS were weakly stained or negative.
Distinct cytoplasmic staining was observed in macrophage while ducal cells in the salivary gland showed strong membranous immunoreactivity. Gastric mucosa, pancreatic islet cells, renal tubules, placental trophoblasts and subsets of neurons displayed moderate cytoplasmic positivity. Additional nuclear staining was for example observed in lymphoid cells and cells in CNS. Remaining normal tissues were in general weakly stained or negative.
Validation
Two (or more) antibodies yielding similar staining patterns which are consistent with available gene/protein characterization data
Two (or more) antibodies yielding similar staining patterns which are consistent with available gene/protein characterization data
Two (or more) antibodies yielding similar staining patterns which are partly consistent with gene/protein characterization data or consistent with limited gene/protein characterization data
