CSNK1G3

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA027010

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA027010 

Host species

Rabbit 

Clonality

pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

6 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment 

Length (aa)

85 

Antigen sequence

MFDYEYDWIGKQLPTPVGAVQQDPALSSNREAHQHRDKMQQSKNQVVSST
NGELNTDDPTAGRSNAPITAPTEVEVMDETNCQKV
 

Matching transcripts

CSNK1G3-003 - ENSP00000423838 [100%]
CSNK1G3-004 - ENSP00000421998 [100%]
CSNK1G3-005 - ENSP00000421385 [100%]
CSNK1G3-009 - ENSP00000429412 [100%]
CSNK1G3-201 - ENSP00000334735 [100%]
CSNK1G3-008 - ENSP00000429914 [95%]
 

Other gene match

 

ANTIBODY VALIDATION

         Immunohistochemistry

Image

 

Description

Immunohistochemical staining of human thyroid gland shows strong cytoplasmic positivity in glandular cells.
More information
 

Retrieval method

HIER pH6 

Antibody dilution

1:15 

Validation IH

Uncertain: One antibody yielding a staining pattern and there is no available gene/protein characterization data or the staining pattern is partly consistent with or contradicted by limited gene/protein characterization data 

         Western Blot

Image

 

Description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: Negative control (vector only transfected HEK293T lysate)
Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY422199)
More information
 

Target mass (kDa)

49.0, 48.9, 40.7, 36.5, 19.2 

Antibody dilution

1:250 

Validation WB

Supportive: Single band corresponding to the predicted size in kDa (+/-20%) 

         Protein array

Image

 

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:500 

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen. 
 

ANTIGEN VIEW

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CSNK1G3-003
 
CSNK1G3-004
 
CSNK1G3-005
 
CSNK1G3-008
 
CSNK1G3-009
 
CSNK1G3-201