The normal tissue page shows antibody staining and, when available, knowledge-based annotated protein expression in 46 human tissues. The assay and annotation is described here.
This page starts with a summary. The summary data can be based on one or more antibodies (reported by the "Antibodies in assay"-field). If the data is based on one single antibody, the IH validation score for that antibody is reported in the "Reliability (Single)"-field (color-coding described here). If the data is based on more than one antibody, the reliability score for the data is reported as "Reliability (APE)" (color-coding described here).
The tissues and cell types can be ordered by organ, cell type or alphabetically.
For each tissue and cell type, the antibody staining is given with the yellow-scale color-coding (described by the scale in the box to the right). Each available antibody is listed in a separate column and the antibody identifier is available at the bottom of the tissue list. The images and annotations can be accessed by clicking on the tissue name.
When available, a knowledge-based annotated protein expression (APE) is given to the right of the antibody staining values. The level of expression is given by a blue-scale color-coding (described by the scale in the box to the right).
At the very bottom of this page, a summary of the staining from each antibody is given, together with a representative image and the immunohistochemistry validation score for the antibody.
Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in neuronal cells.
Immunohistochemical staining of human kidney shows moderate cytoplasmic positivity in cells in tubule.
Immunohistochemical staining of human cerebellum shows strong nuclear positivity in the granular layer while Purkinje cells were moderately stained in the cytoplasm.
Summary
Strong nuclear staining in combination with moderate cytoplasmic positivity was observed in granular layer of cerebellum, syncytiotrophoblasts of placenta and in glandular epithelia of the gastro-intestinal tract. Some neuronal cells were also distinctly stained. Remaining normal tissues were in general negative.
Most normal cells displayed weak to moderate cytoplasmic staining. Cells in the granular layer of cerebellum displayed moderate nuclear positivity. Cells in lymphoid tissues and placenta were generally negative.
A majority of normal cells showed weak to moderate cytoplasmic staining. Subsets of cells in CNS and non-germinal center cells in lymphoid tissues were strongly stained. Squamous epithelia, breast, prostate, glial cell and cells in the female genital tract were negative.
Validation
Two (or more) antibodies yielding similar staining patterns which are consistent with available gene/protein characterization data
Two (or more) antibodies yielding similar staining patterns which are consistent with available gene/protein characterization data
Two (or more) antibodies yielding similar staining patterns which are consistent with available gene/protein characterization data
