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SUBCELLULAR LOCATION

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Main location(s)

Nucleus, Nucleoli, Nucleus but not nucleoli

Additional location(s)

 

Staining summary

Localized to the nucleus and nucleoli.

Reliability (APE)

Medium

Antibodies in assay

CAB004347, HPA011753, HPA017206
 

Antibody CAB004347

 

Antibody HPA011753

 

Antibody HPA017206

 

         Cell line A-431

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Location(s)

  Nucleoli, Nucleus but not nucleoli  

Staining characteristics

  Cluster  

Staining intensity

  Moderate Negative 

Validation

  
One/multiple location(s) supported by experimental gene/protein characterization data.
 
No staining.
 

Comment

    

         Cell line U-2 OS

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Location(s)

 Nucleoli, Nucleus but not nucleoli  

Staining characteristics

 Cluster  

Staining intensity

Negative Moderate Negative 

Validation

No staining.
 
One/multiple location(s) supported by experimental gene/protein characterization data.
 
No staining.
 

Comment

   

         Cell line U-251 MG

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Location(s)

 Nucleoli, Nucleus but not nucleoli  

Staining characteristics

 Cluster  

Staining intensity

Negative Moderate Negative 

Validation

No staining.
 
One/multiple location(s) supported by experimental gene/protein characterization data.
 
No staining.
 

Comment

   

         Antibody summary


Immunofluorescent staining of human cell line U-251 MG shows no positivity.
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleoli & nucleus but not nucleoli.
 

Immunofluorescent staining of human cell line U-2 OS shows no positivity.
 

Summary

No staining was observed in any of the three cell lines.   Staining of nucleus and nucleoli in all three cell lines.   No staining was observed in any of the three cell lines.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is supported by literature.
 
The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Nucleoli
Nucleus
Nucleus but not nucleoli