CARHSP1

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA051911

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA051911 

Host species

Rabbit 

Clonality

pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

10 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment 

Length (aa)

31 

Antigen sequence

MSSEPPPPPQPPTHQASVGLLDTPRSRERSP
 

Matching transcripts

CARHSP1-001 - ENSP00000379838 [100%]
CARHSP1-002 - ENSP00000311847 [100%]
CARHSP1-004 - ENSP00000455016 [100%]
CARHSP1-006 - ENSP00000457864 [100%]
CARHSP1-007 - ENSP00000455284 [100%]
CARHSP1-008 - ENSP00000455522 [100%]
CARHSP1-010 - ENSP00000456909 [100%]
CARHSP1-011 - ENSP00000457213 [100%]
CARHSP1-012 - ENSP00000455855 [100%]
CARHSP1-015 - ENSP00000460812 [100%]
 

Other gene match

 

ANTIBODY VALIDATION

         Immunohistochemistry

Image

 

Description

Immunohistochemical staining of human placenta shows strong cytoplasmic and nuclear positivity in trophoblastic cells.
More information
 

Retrieval method

HIER pH6 

Antibody dilution

1:60 

Validation IH

Uncertain: One antibody yielding a staining pattern and there is no available gene/protein characterization data or the staining pattern is partly consistent with or contradicted by limited gene/protein characterization data 

         Western Blot

Image

 

Description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT-4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

15.9, 14.2, 13.7, 13.4, 12.1, 10.1, 7.7 

Antibody dilution

1:190 

Validation WB

Supportive: Single band corresponding to the predicted size in kDa (+/-20%) 

         Protein array

Image

 

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:1500 

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen. 
 

ANTIGEN VIEW

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CARHSP1-001
 
CARHSP1-002
 
CARHSP1-004
 
CARHSP1-006
 
CARHSP1-007
 
CARHSP1-008
 
CARHSP1-010
 
CARHSP1-011
 
CARHSP1-012
 
CARHSP1-015