The normal tissue page shows antibody staining and, when available, knowledge-based annotated protein expression in 46 human tissues. The assay and annotation is described here.
This page starts with a summary. The summary data can be based on one or more antibodies (reported by the "Antibodies in assay"-field). If the data is based on one single antibody, the IH validation score for that antibody is reported in the "Reliability (Single)"-field (color-coding described here). If the data is based on more than one antibody, the reliability score for the data is reported as "Reliability (APE)" (color-coding described here).
The tissues and cell types can be ordered by organ, cell type or alphabetically.
For each tissue and cell type, the antibody staining is given with the yellow-scale color-coding (described by the scale in the box to the right). Each available antibody is listed in a separate column and the antibody identifier is available at the bottom of the tissue list. The images and annotations can be accessed by clicking on the tissue name.
When available, a knowledge-based annotated protein expression (APE) is given to the right of the antibody staining values. The level of expression is given by a blue-scale color-coding (described by the scale in the box to the right).
At the very bottom of this page, a summary of the staining from each antibody is given, together with a representative image and the immunohistochemistry validation score for the antibody.
Mainly cytoplasmic and membranous expression in a subset of tissues including kidney tubules, hepatocytes and glandular cells in the gastrointestinal tract.
Immunohistochemical staining of human kidney shows strong nuclear and cytoplasmic positivity in cells of tubules.
Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules.
Summary
Proximal renal tubules displayed strong positivity in luminal membranes and cytoplasm. The intestine and epididymis showed positivity in luminal membranes. Skeletal muscle showed moderate cytoplasmic staining while smooth muscle displayed weak positivity. Remaining normal tissues were predominantly negative.
Strong cytoplasmic positivity was observed in hepatocytes, exocrine pancreas, renal tubules and salivary gland. Moderate luminal membranous and occasional cytoplasmic positivity was observed in intestinal tract. A fraction of cells in lymphoid tissues were moderately stained. Remaining normal tissues were mainly negative.
Validation
Two (or more) antibodies yielding similar or partly similar staining patterns and there is no available gene/protein characterization data
Two (or more) antibodies yielding similar or partly similar staining patterns and there is no available gene/protein characterization data
