A protein array containing 384 different antigens including the antibody target is used to analyse antibody specificity. Depending on the array interaction profile the antibody is scored as supportive, uncertain or unreliable.
For each antibody, the observed staining in the different cell lines is assigned a validation score based on concordance with available experimental gene/protein characterization data in the UniProtKB/Swiss-Prot database. The validation scores for up to three cell lines are merged into one of the main categories; Supportive, Uncertain, or Unreliable, to represent the overall antibody staining in all analyzed cell lines.
Immunofluorescent staining of human cell line SiHa shows localization to nucleoplasm & cytosol.
The immunostaining patterns is compared for consistency with available experimental gene/protein characterization data in the UniProtKB/Swiss-Prot database and other experimental evidence for location described in scientific literature.