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SUBCELLULAR LOCATION

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Main location(s)

Nucleus, Cytoplasm

Additional location(s)

 

Staining summary

Localized to the cytoplasm and/or the nucleus.

Reliability (APE)

Very low

Antibodies in assay

CAB017815, HPA018793, HPA018816
 

Antibody CAB017815

 

Antibody HPA018793

 

Antibody HPA018816

 

         Cell line A-431

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Location(s)

  Nucleus but not nucleoli Cytoplasm 

Staining characteristics

   Granular 

Staining intensity

  Strong Moderate 

Validation

  
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 

Comment

    

         Cell line U-2 OS

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Location(s)

Cytoplasm Nucleus but not nucleoli   

Staining characteristics

    

Staining intensity

Weak Moderate   

Validation

Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
   

Comment

    

         Cell line U-251 MG

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Location(s)

Nucleus, Cytoplasm Nucleus but not nucleoli Cytoplasm 

Staining characteristics

Spotty  Granular 

Staining intensity

Strong Moderate Moderate 

Validation

Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 

Comment

   

         Antibody summary


Immunofluorescent staining of human cell line U-2 OS shows positivity in cytoplasm.
 

Immunofluorescent staining of human cell line A-431 shows positivity in nucleus but not nucleoli.
 

Immunofluorescent staining of human cell line A-431 shows positivity in cytoplasm.
 

Summary

Staining of cytoplasm in U-251 MG and U-2 OS with additional staining of nucleus in U-251 MG.   Staining of nucleus in all three cell lines.   Staining of cytoplasm in U-251 MG and A-431  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Cytoplasm
Nucleus