Fields »

SUBCELLULAR LOCATION

? »

Main location(s)

Nucleus but not nucleoli, Plasma membrane, Cytoplasm

Additional location(s)

 

Staining summary

Localized to the cytoplasm, nucleus and plasma membrane.

Reliability (APE)

Low

Antibodies in assay

HPA019497, HPA021186
 

Antibody HPA019497

 

Antibody HPA021186

 

         Cell line A-431

Toggle channels

 
 

Location(s)

Nucleus but not nucleoli Nucleus but not nucleoli, Plasma membrane, Cytoplasm 

Staining characteristics

 Spotty 

Staining intensity

Moderate Strong 

Validation

Location not consistent with experimental gene/protein characterization data.
 
Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 

Comment

  

         Cell line U-2 OS

Toggle channels

  
 

Location(s)

  Plasma membrane, Cytoplasm 

Staining characteristics

   

Staining intensity

  Strong 

Validation

  
Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 

Comment

   

         Cell line U-251 MG

Toggle channels

 
 

Location(s)

Nucleus but not nucleoli Nucleus but not nucleoli, Plasma membrane, Cytoplasm 

Staining characteristics

 Spotty 

Staining intensity

Moderate Moderate 

Validation

Location not consistent with experimental gene/protein characterization data.
 
Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line A-431 shows positivity in nuclei but not nucleoli.
 

Immunofluorescent staining of human cell line A-431 shows positivity in nucleus but not nucleoli, plasma membrane & cytoplasm.
 

Summary

Staining of nuclei but not nucleoli in U-251 MG and A-431.   Staining of plasma membrane/cytoplasm in all three cell lines with additional staining of spots in nucleus in U-251 MG and A-431.  

Validation

The subcellular location is not consistent with literature.
 
The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Cytoplasm
Nucleus but not nucleoli
Plasma membrane