Fields »

SUBCELLULAR LOCATION

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Main location(s)

Nucleus, Plasma membrane, Cell Junctions

Additional location(s)

Nucleus but not nucleoli 

Staining summary

Mainly localized to the plasma membrane and cell junctions but also to the nucleus.

Reliability (APE)

High

Antibodies in assay

CAB005103, HPA030411
 

Antibody CAB005103

 

Antibody HPA030411

 

         Cell line A-431

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Location(s)

   

Staining characteristics

   

Staining intensity

Negative   

Validation

No staining.
   

Comment

   

         Cell line U-2 OS

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Location(s)

Plasma membrane, Cell Junctions Nucleus but not nucleoli, Plasma membrane, Cell Junctions 

Staining characteristics

  

Staining intensity

Weak Moderate 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data.
 
Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

  

         Cell line U-251 MG

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Location(s)

  Nucleus but not nucleoli, Cell Junctions 

Staining characteristics

   

Staining intensity

  Strong 

Validation

  
Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

   

         Antibody summary


Immunofluorescent staining of human cell line U-2 OS shows positivity in plasma membrane & cell junctions.
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus but not nucleoli, plasma membrane & cell junctions.
 

Summary

Staining of plasma membrane and cell junctions in U-2 OS.   Staining of nucleus and cell junctions in U-251 MG and U-2 OS with additional staining of plasma membrane in U-2 OS.  

Validation

The subcellular location is supported by literature.
 
The subcellular location is supported by literature.
 

Dictionary

Cell junctions
Nucleus
Nucleus but not nucleoli
Plasma membrane