Fields »

SUBCELLULAR LOCATION

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Main location(s)

Nucleus but not nucleoli

Additional location(s)

 

Staining summary

Localized to the nucleus but excluded from nucleoli.

Reliability (APE)

High

Antibodies in assay

HPA003258, CAB004411, HPA028791
 

Antibody HPA003258

 

Antibody CAB004411

 

Antibody HPA028791

 

         Cell line A-431

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Location(s)

Nucleus but not nucleoli Nucleus but not nucleoli Nucleus but not nucleoli 

Staining characteristics

Granular   

Staining intensity

Moderate Weak Moderate 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 

Comment

   

         Cell line U-2 OS

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Location(s)

Nucleus but not nucleoli  Nucleus but not nucleoli 

Staining characteristics

Granular   

Staining intensity

Moderate Negative Moderate 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 
No staining.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 

Comment

   

         Cell line U-251 MG

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Location(s)

Nucleus but not nucleoli   Nucleus but not nucleoli 

Staining characteristics

Granular    

Staining intensity

Moderate   Moderate 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
   
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 

Comment

    

         Antibody summary


Immunofluorescent staining of human cell line U-251MG shows positivity in nucleus but not nucleoli.
 

Immunofluorescent staining of human cell line A-431 shows positivity in nucleus but not nucleoli.
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus but not nucleoli.
 

Summary

Staining of nuclei in all three cell lines.   Staining of nucleus in A-431.   Staining of nucleus in all three cell lines.  

Validation

The subcellular location is supported by literature.
 
The subcellular location is supported by literature.
 
The subcellular location is supported by literature.
 

Dictionary

Nucleus but not nucleoli