Fields »

SUBCELLULAR LOCATION

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Main location(s)

Nucleus but not nucleoli, Cytoplasm

Additional location(s)

 

Staining summary

Localized to the cytoplasm and nucleus.

Reliability (APE)

High

Antibodies in assay

HPA005661, CAB025996
 

Antibody HPA005661

 

Antibody CAB025996

 

         Cell line A-431

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Location(s)

Cytoplasm Nucleus but not nucleoli, Cytoplasm 

Staining characteristics

  

Staining intensity

Moderate Weak 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but supported by ≥1 other antibody.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but supported by ≥1 other antibody.
 

Comment

  

         Cell line U-2 OS

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Location(s)

   

Staining characteristics

   

Staining intensity

Negative   

Validation

No staining.
   

Comment

   

         Cell line U-251 MG

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Location(s)

Nucleus but not nucleoli   

Staining characteristics

Granular   

Staining intensity

Weak   

Validation

One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but supported by ≥1 other antibody.
   

Comment

   

         Antibody summary


Immunofluorescent staining of human cell line U-251 MG shows positivity in nucleus but not nucleoli.
 

Immunofluorescent staining of human cell line A-431 shows positivity in nucleus but not nucleoli & cytoplasm.
 

Summary

Staining of cytoplasm in A-431 and nucleus in U-251 MG.   Staining of nucleus and cytoplasm in all three cell lines.  

Validation

The subcellular location is supported by literature.
 
The subcellular location is supported by literature.
 

Dictionary

Cytoplasm
Nucleus but not nucleoli