Fields »

SUBCELLULAR LOCATION

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Main location(s)

Cytoplasm

Additional location(s)

Nucleus but not nucleoli, Cytoskeleton (Actin filaments) 

Staining summary

Mainly localized to the cytoplasm.

Reliability (APE)

Medium

Antibodies in assay

HPA001114, HPA042505
 

Antibody HPA001114

 

Antibody HPA042505

 

         Cell line A-431

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Location(s)

  Nucleus but not nucleoli, Cytoplasm 

Staining characteristics

   

Staining intensity

  Weak 

Validation

  
The multi-targeting antibody yielding a staining pattern with no available gene/protein characterization data.
 

Comment

   

         Cell line U-2 OS

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Location(s)

Cytoplasm Cytoplasm, Cytoskeleton (Actin filaments) 

Staining characteristics

Granular  

Staining intensity

Weak Weak 

Validation

Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 
The multi-targeting antibody yielding a staining pattern with no available gene/protein characterization data.
 

Comment

  

         Cell line U-251 MG

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Location(s)

  Nucleus but not nucleoli, Cytoplasm 

Staining characteristics

  Granular 

Staining intensity

  Weak 

Validation

  
The multi-targeting antibody yielding a staining pattern with no available gene/protein characterization data.
 

Comment

   

         Antibody summary


Immunofluorescent staining of human cell line U-2 OS shows positivity in cytoplasm.
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in cytoplasm & cytoskeleton (actin filaments).
 

Summary

Staining of cytoplasm in U-2OS.   Staining of cytoplasm in all three cell lines with additional staining of nucleus in A-431 and U-251 MG and cytoskeleton (actin filaments) in U-2 OS.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Cytoplasm
Actin filaments
Nucleus but not nucleoli