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SUBCELLULAR LOCATION

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Main location(s)

Nucleus but not nucleoli, Cytoplasm

Additional location(s)

 

Staining summary

Localized to the nucleus and cytoplasm.

Reliability (APE)

Very low

Antibodies in assay

HPA007326, HPA026088
 

Antibody HPA007326

 

Antibody HPA026088

 

         Cell line A-431

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Location(s)

 Nucleus but not nucleoli, Cytoplasm 

Staining characteristics

  

Staining intensity

Negative Weak 

Validation

No staining.
 
Location not consistent with experimental gene/protein characterization data.
 

Comment

  

         Cell line U-2 OS

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Location(s)

 Nucleus but not nucleoli, Cytoplasm 

Staining characteristics

  

Staining intensity

Negative Moderate 

Validation

No staining.
 
Location not consistent with experimental gene/protein characterization data.
 

Comment

  

         Cell line U-251 MG

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Location(s)

 Nucleus but not nucleoli, Cytoplasm 

Staining characteristics

  

Staining intensity

Negative Weak 

Validation

No staining.
 
Location not consistent with experimental gene/protein characterization data.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line U-251MG shows no positivity.
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus but not nucleoli & cytoplasm.
 

Summary

No staining was observed in any of the three cell lines.   Staining of nucleus and cytoplasm in all three cell lines.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is not consistent with literature.
 

Dictionary

Cytoplasm
Nucleus but not nucleoli