The normal tissue page shows antibody staining and, when available, knowledge-based annotated protein expression in 46 human tissues. The assay and annotation is described here.
This page starts with a summary. The summary data can be based on one or more antibodies (reported by the "Antibodies in assay"-field). If the data is based on one single antibody, the IH validation score for that antibody is reported in the "Reliability (Single)"-field (color-coding described here). If the data is based on more than one antibody, the reliability score for the data is reported as "Reliability (APE)" (color-coding described here).
The tissues and cell types can be ordered by organ, cell type or alphabetically.
For each tissue and cell type, the antibody staining is given with the yellow-scale color-coding (described by the scale in the box to the right). Each available antibody is listed in a separate column and the antibody identifier is available at the bottom of the tissue list. The images and annotations can be accessed by clicking on the tissue name.
When available, a knowledge-based annotated protein expression (APE) is given to the right of the antibody staining values. The level of expression is given by a blue-scale color-coding (described by the scale in the box to the right).
At the very bottom of this page, a summary of the staining from each antibody is given, together with a representative image and the immunohistochemistry validation score for the antibody.
Selective cytoplasmic and membranous expression in a few cell types including basal cells in skin, molecular layer cells cerebellum and subsets of inflammatory cells in stroma.
Immunohistochemical staining of human cerebellum shows moderate cytoplasmic positivity in cells in molecular layer.
Immunohistochemical staining of human breast shows strong cytoplasmic and membranous positivity in glandular cells.
Summary
A subset of inflammatory cells and basal cells of epidermis were distinctly positive in the cytoplasm. Molecular layer of cerebellum were moderately stained. Remaining normal tissues were mainly negative.
Breast epithelium and purkinje cells showed strong membranous positivity. A fraction of cells in basal layer of squamous epithelium displayed strong cytoplasmic positive. Moderate staining was observed in lung macrophages, a subset of renal tubular cells, cells in seminiferus ducts and falopian tube. Remaining normal tissues were in general weakly stained or negative.
Validation
Two (or more) antibodies yielding similar staining patterns which are consistent with available gene/protein characterization data
Two (or more) antibodies yielding similar staining patterns which are consistent with available gene/protein characterization data
