AASDH

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA036472

 

Antibody HPA036473

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA036472 HPA036473 

Host species

Rabbit Rabbit 

Clonality

pAb pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

12 7 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment Recombinant protein fragment 

Length (aa)

94 92 

Antigen sequence

LGRKDSQIKRHGKRLNIELVQQVAEELQQVESCAVTWYNQEKLILFMVSK
DASVKEYIFKELQKYLPSHAVPDELVLIDSLPFTSHGKIDVSEL
 
RKLSDINQEEASGTSLHQKAIMTFTCHNEINAFVVLSRGSQILSLNSTRF
LTKLGHCSSACPSDSVSQTNIQNLKGLNSPVLIGKSKDPSCV
 

Matching transcripts

AASDH-001 - ENSP00000205214 [100%]
AASDH-002 - ENSP00000423760 [100%]
AASDH-006 - ENSP00000421171 [100%]
AASDH-202 - ENSP00000409656 [100%]
AASDH-203 - ENSP00000473564 [100%]
 AASDH-001 - ENSP00000205214 [100%]
AASDH-002 - ENSP00000423760 [100%]
AASDH-006 - ENSP00000421171 [100%]
AASDH-201 - ENSP00000392158 [100%]
AASDH-202 - ENSP00000409656 [100%]
AASDH-203 - ENSP00000473564 [100%]
 

Other gene match

  

ANTIBODY VALIDATION

         Immunohistochemistry

Image

  

Description

Immunohistochemical staining of human kidney shows strong granular cytoplasmic positivity in renal tubules.
More information
 Immunohistochemical staining of human liver shows strong cytoplasmic and membranous positivity in hepatocytes.
More information
 

Retrieval method

HIER pH6 HIER pH6 

Antibody dilution

1:50 1:75 

Validation IH

Uncertain: Two (or more) antibodies yielding dissimilar or partly similar staining patterns and there is no available gene/protein characterization data or only limited gene/protein characterization data Uncertain: Two (or more) antibodies yielding dissimilar or partly similar staining patterns and there is no available gene/protein characterization data or only limited gene/protein characterization data 

         Western Blot

Image

  

Description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT-4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

122.6, 111.4, 96.2, 94.3, 89.1 122.6, 111.4, 96.2, 94.3, 89.1, 67.9 

Antibody dilution

1:250 1:250 

Validation WB

Non-supportive: Only bands not corresponding to the predicted size Uncertain: Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data 

         Protein array

Image

  

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:500 1:500 

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen. Uncertain: Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%). 
 

ANTIGEN VIEW

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AASDH-001
 
AASDH-002
 
AASDH-006
 
AASDH-201
 
AASDH-202
 
AASDH-203