Fields »

SUBCELLULAR LOCATION

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Main location(s)

Nucleus but not nucleoli

Additional location(s)

Plasma membrane, Cytoplasm 

Staining summary

Mainly localized to nucleus but also cytoplasm and plasma membrane.

Reliability (APE)

Medium

Antibodies in assay

HPA019907, HPA019943
 

Antibody HPA019907

 

Antibody HPA019943

 

         Cell line A-431

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Location(s)

Nucleus but not nucleoli Nucleus but not nucleoli, Cytoplasm 

Staining characteristics

 Granular 

Staining intensity

Strong Moderate 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 
One/multiple location(s) with no available experimental gene/protein characterization data.
 

Comment

  

         Cell line U-2 OS

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Location(s)

Nucleus but not nucleoli Nucleus but not nucleoli, Cytoplasm 

Staining characteristics

 Spotty 

Staining intensity

Strong Moderate 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 
One/multiple location(s) with no available experimental gene/protein characterization data.
 

Comment

  

         Cell line U-251 MG

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Location(s)

Nucleus but not nucleoli, Plasma membrane Nucleus but not nucleoli, Cytoplasm 

Staining characteristics

  

Staining intensity

Strong Moderate 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 
One/multiple location(s) with no available experimental gene/protein characterization data.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line U-251MG shows positivity in nucleus but not nucleoli & plasma membrane.
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus but not nucleoli & cytoplasm.
 

Summary

Staining of nucleus in all three cell lines with additional staining of plasma membrane in U-251 MG.   Staining of nucleus and cytoplasm in all three cell lines.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Cytoplasm
Nucleus but not nucleoli
Plasma membrane