The normal tissue page shows antibody staining and, when available, knowledge-based annotated protein expression in 46 human tissues. The assay and annotation is described here.
This page starts with a summary. The summary data can be based on one or more antibodies (reported by the "Antibodies in assay"-field). If the data is based on one single antibody, the IH validation score for that antibody is reported in the "Reliability (Single)"-field (color-coding described here). If the data is based on more than one antibody, the reliability score for the data is reported as "Reliability (APE)" (color-coding described here).
The tissues and cell types can be ordered by organ, cell type or alphabetically.
For each tissue and cell type, the antibody staining is given with the yellow-scale color-coding (described by the scale in the box to the right). Each available antibody is listed in a separate column and the antibody identifier is available at the bottom of the tissue list. The images and annotations can be accessed by clicking on the tissue name.
When available, a knowledge-based annotated protein expression (APE) is given to the right of the antibody staining values. The level of expression is given by a blue-scale color-coding (described by the scale in the box to the right).
At the very bottom of this page, a summary of the staining from each antibody is given, together with a representative image and the immunohistochemistry validation score for the antibody.
Immunohistochemical staining of human smooth muscle shows strong cytoplasmic positivity in smooth muscle cells.
Immunohistochemical staining of human heart muscle shows strong cytoplasmic and membranous positivity in myocytes.
Immunohistochemical staining of human stomach, upper shows strong membranous positivity in glandular cells.
Immunohistochemical staining of human stomach, upper shows strong membranous positivity in glandular cells.
Summary
Strong cytoplasmic positivity was observed in smooth muscle and stomach. Distinct staining of luminal membranes and cilium was observed in epididymis, the intesteine, renal tubules, exocrine pancreas and trophoblastic cells. Lymphoid cells in reaction centra showed mocerate to strong cytoplasmic staining. Remaining normal tissues were mainly negative.
Strong cytoplasmic and membranous staining was observed in cardiac myocytes. Strong positivity was also observed in subsets of smooth and skeletal muscle cells. Remaining normal tissues were in general negative.
Most normal tissues showed moderate to strong membranous and/or cytoplasmic positivity. Hepatocytes, Purkinje cells and glial cells were negative.
Gastrointestinal tract and epididymis displayed strong membranous positivity. Myoepithelial cells and lymphoid tissue exhibited strong cytoplasmic staining. Remaining normal tissues were weakly stained or negative.
Validation
Two (or more) antibodies yielding partly similar staining patterns which are partly consistent with gene/protein characterization data or consistent with limited gene/protein characterization data
Two (or more) antibodies yielding partly similar staining patterns which are consistent with gene/protein characterization data
The multi-targeting antibody yielding a staining pattern consistent with gene/protein characterization data for at least one of the genes whereas any of the other genes has no available gene/protein characterization data or is contradicted by gene/protein
The multi-targeting antibody yielding a staining pattern consistent with gene/protein characterization data for at least one of the genes whereas any of the other genes has no available gene/protein characterization data or is contradicted by gene/protein
